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KMID : 1145720190070020137
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Korean Herbal Medicine Informatics
2019 Volume.7 No. 2 p.137 ~ p.145
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Quantitative Analysis of Schizandrin and Gomisin A in Traditional Herbal Formula, Saengmaek-san
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Seo Chang-Seob
Kim Jung-Hoon
Shin Hyeun-Kyoo
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Abstract
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Saengmaek-san (SMS) comprises three herbal medicines Liriopis seu Ophiopogonis Tuber, Ginseng Radix, and Schisandrae Fructus. SMS has been traditionally used for the treatment of ischemic disease in Asia. In this study, an analytical method using high-performance liquid chromatography (HPLC) and a photodiode array detector (PDA) (HPLC?PDA) was used for the quantitative analysis of schizandrin and gomisin A in SMS, a traditional herbal formula. Chromatographic separation of the two compounds was carried out using a Gemini C18 column and two mobile phases, distilled water and acetonitrile, with gradient elution. The coefficient of determination of the calibration curve for the quantitative analysis of the marker components of Schisandrae Fructus in an SMS sample, schizandrin and gomisin A, was 1.0000. The limit of detection of the marker components was 3.28 mg/mL and 3.19 mg/mL, and the limit of quantification was 9.93 mg/mL and 9.67 mg/mL. Recovery and intra- and interday precisions were 95.44-103.58% and < 1.5%, respectively. The quantities of schizandrin and gomisin A were 0.244 and 0.028 mg/freeze-dried g, respectively. The optimized HPLC?PDA method could be expected to provide basic data for the quality control of SMS and other related herbal formulas.
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KEYWORD
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Quantitative Analysis, Saengmaek-san, HPLCPDA
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